Rationale: perform plaque assay for the sample enriched from Melissa’s soil and also the enriched sample from R&L experiment, soil sample 2

Process:

1. washed table and setup aseptic zone
2. Made LB agar media for 3 plates
   • 6 mL LB broth
   • 68 µL 1 M CaCl2
   • 7.5 mL 2X TA
3. added 30 µL lysate (Melissa’s soil and R&L SS2) each to 0.5 mL arthro and let sit for 15 minutes
4. Poured control plate with ~5mL from the LB agar media and divided the remaining agar into 2 tubes
5. Added arthro lysate mixture to each LB agar media tube
6. Poured plates from tubes and let sit for ~15 minutes
7. Incubated for 48 hours at 28 degrees Celsius

Next Steps: Another plaque assay but with alterations to get a higher titer.