Rationale: I now want to purify my phage so I will pick a plaque and run a plaque assay on it to try to get a single type of phage.

Procedure:

1. Marked a single plaque in my spot test.
2. Obtained a microcentrifuge tube and placed 100µL of phage buffer inside.
3. Stuck a micro-pipette tip into marked plaque and swirled around. a small bit trying to avoid arthrobacter.
4. Stuck tip into phage buffer and swirled around to dislodge phage particles.
5. Diluted twice by taking 10µL of next most dilute sample and placing it into 90µL of phage buffer.
6. Enriched each dilution for 30 minutes in 0.5mL of arthrobacter.
7. Made top agar by mixing 8mL of LB Broth, 90µL of CaCl2, and 10mL of 2XTA then plated 4.5mL as a control.
8. Put 4.5mL of top agar into each tube of arthro bacter then plated 10^0 then 10^-2 dilution.
9. Accidentally plated 10^-1 on top of 10^-2 dilution.
10. Inverted both the other plates and placed incubator for 48 hours.
11. Kept 10^-2 dilution upright and placed in incubator for 48 hours.

Obsercations: I will update observations when I go to open lab this Friday.

Conclusions and Next Steps: Next I need to try to web a plate and obtain a high titer. Once I web a plate I can flood it and get a lot of high titer lysate which I can use to do DNA sequencing and other important procedures.