Rationale:

“KEA 11/14/18 Ferranti DNA Ex.” conical vial will be centrifuged and the supernatant will be decanted in order to isolate the phage particles for DNA Extraction.

Procedures:

1. Centrifuged “KEA 11/14/18 Ferranti DNA Ex.” conical vial at 10,000g for 27 minutes at 4ºC.
2. Decanted supernatant from the centrifuged conical vial.
3. Stored pellet in the refrigerator.

Observations:

• At first, the “KEA 11/14/18 Ferranti DNA Ex.” conical vial was centrifuged for only 20 minutes. There was uncertainty whether or not the phage particles separated completely, so the “KEA 11/14/18 Ferranti DNA Ex.” conical vial was centrifuged an additional 7 minutes.
• The supernatant was a light shade of yellow and the phage pellet was a light tannish color. The pellet stuck to the side of the conical vial. The pellet was relatively small. The picture below shows both the phage pellet and supernatant.

Next Steps:

Ferranti will be viewed under a transmission electron microscope. If there is enough time, DNA extraction will be completed.