Rationale: The purpose of this lab is to perform serial dilutions to calculate the titer of the lysate.

Description of Procedures:

1. The workstation was cleaned using aseptic technique and an aseptic zone was created with an ethanol burner.
2. Serial dilutions were performed out to a dilution of 10^-5.
3. 10 ul of each dilution was added to 0.5 ml of arthrobacter and allowed to sit for 10 minutes.
4. Top agar was made for six plates. 12 ml of LB and 135 ul of CaCl2 were added to a tube.
5. 15 ml of 2 x TA were added to the tube. 4,5 ml of top agar solution was poured onto a plate labeled LIP 11-12-18 Control. 4.5 ml was mixed with the arthrobacter for each dilution and poured onto five plates labeled LIP 10-12-18 10^-1, LIP 10-12-18 10^-2, LIP 10-12-18 10^- 3, LIP 10-12-18 10^-4 , LIP 10-12-18 10^-5.
6. Plates were allowed to sit for 10 minutes and then inverted and stored in the incubator until the next lab.
7. The workstation was cleaned using aseptic technique and materials were properly stored and disposed of.

Observations:

• No 10^0 plate was made.
• Bubbles were seen on all plates.

Interpretations/Next Steps:
The procedure was complete. The next step will be to use the serial dilutions to calculate the titer of the lysate.

Plates: