11/9/18

Rational:

To do  a plaque assay in order to determine the titer of the new lysate.

Procedure:

• Cleaned the lab desk
• Filtered  the lysate from the 5 flooded plates
• Put 4 mL of LB broth into a tube
• Added 10 ML of lysate to .5 mL arthro and waited 10 min
• Added 45 ML of CaCl2 to the TA
• Added 2 ml of TA mixture to the .5 mL arthro
• Added 2.5 mL of TA to the control and arthro  and poured on the plates
• Waited 15 min
• Put in the incubator at 26 C at 4

Conclusion:

Next lab we will calculate the titer of the lysate and do a TEM on the lysate if the titer is high enough. If the titer is not high enough then we will get a new lysate with a higher titer.