Rationale: Lauren and I decided to continue searching for phage presence in the soil on the basis of soil texture. We will collect different soil samples then run spot tests and sand silt clay experiments to see if there is any correlation.

Process:

1. Wash table and set up aseptic zone
2. Added ~10 mL LB broth to soil (filled to 2 mL mark)
3. Shook and vortexed for 15 minutes
4. Centrifuged for 10 minutes at 3000 G
5. Filtered with syringe filter
   1. ended with ~7 mL for enriched isolation and ~1 mL for direct isolation
6. Added 0.5 mL arthro to enriched isolation
7. Incubated enriched for 48 hours at 28 degrees C
8. Stored direct in fridge

LEF – soil sample 1

RSM – soil sample 2

Next steps: Perform spot test to check for any plaques.