Rationale: After TEM imaging and determining the titer wasn’t high enough, will be continuing to raise the titer strength to easily image the phages in the lysate

Procedure:

• Created an aseptic zone to prevent contamination of plates
• Obtained the lysate from the refrigerator, as well as 4 agar plates
• Created a 10^-1 dilution and 10^-2 dilution
  • The 10^-1 was created by adding 10μL of lysate to 90μL of PB; the 10^-2 was created by adding 10μL of the 10^-1 to 90μL of PB
• Added 10μL of each lysate into 0.5mL of arthrobacter (Respectively)
• Obtained a 50mL conical vial and added in 8mL LB broth, 10mL 2xTA and 90μL CaCl2
• Immediately added 4.5mL into each arthrobacter + lysate vial (Respectively) and plated
• Allowed the plates to sit for 15 minutes and moved to incubation

Observations:

• The rest of the team was working to calculate the titer of a plaque assay and calculated the amount of lysate needed to web a plate; we are using the same lysate and will be working together to continue to raise the strength of the titer

Next Steps/Conclusion: Next lab, will check the results of the plaque assays and hopefully will be able to count plaques and determine the titer strength and determine the amount of lysate needed to web a plate.