10/29/18

Rational:

To collect, filter, and collect metadata on a new soil sample in order to perform PCR and gel elctrophoresis on that soil sample.

Procedure:

• Collected soil 2 ft from a Chestnut Oak in the Earle Courtyard
• Measured trunk circumference 137 cm from the ground
• Measured the tree height and canopy width
• Cleaned lab desk with CiDecon and ethanol
• Put 2 mL of soil G into a tube
• Added 10 mL of LB broth and shook for 15 min
• Weighed empty weigh boat and weigh boat with soil G then put under hood
• Put soil in pH vial and added Di water and shook
• tested the pH
• Centrifuged soil G for 10 min at 3,000 G
• Put 10 mL of soil into a tube
• Added 20 mL of DI water and 3 drops of soil dispersion
• Shook the tube for 45 sec and put under hood
• Filtered soil G and added .5 mL arthro
• Put in shaking incubator at 4:15-2:30 at 26 C

Observations:

• Tree height- 666.9 cm
• Trunk circumference- 44.4 cm
• Canopy width- 167.6 cm
• Weigh boat- 1.99 g
• Weigh boat with soil- 4.32 g
• Wet soil- 2.33 g
• pH- 6.5

Conclusion:

The results for soil F were inconclusive so a new gel elctrophoresis will be done, but another soil sample has been prepared for PCR so that a gel elctrophoresis can be done on it at the same time as soil F. Next lab I will do PCR on soil G.

Fig.1.G – The Chestnut Oak found in the Earle courtyard. Note there are two Chestnut Oaks in close proximity, however the other is significantly more healthy.

Fig.2.G – There are several very yellow leaves as shown in this image.

Fig.3.G – The leaf size and shape of this tree which identifies it as a Chestnut Oak.

Fig.4.G – This image show the pH of soil G is 6.5 as seenby the color of the pH paper.