Rationale: Complete spot test with new sample isolations and the re-purified products from the heating and killing bacteria method to check for plaques.

Process:

1. Set up aseptic system
2. Made LB agar media for 1 control plate and 2 sample plates

   1. Reagents	Control	Sample
      LB Broth	2 mL	4 mL
      2x TA	2.5 mL	5 mL
      CaCl2	23 µL	45 µL
      arthro	NA	1 mL

3. poured 5 mL of LB agar media into each plate and let sit ~5 min
4. filtered enriched sample with syringe filter
5. pipetted 5 µL of direct, enriched, RSM1, SJ1, LCG1, RSM2, SJ2, LCG2, and phage buffer (negative control) into designated areas of sample plate and let sit ~10 min
   1. the initialed samples are the results from the heating and killing bacteria method
   2. the 1 indicates arthro was added and the 2 indicates arthro was not added (except in the case of LCG2, where arthro was added)
6. incubated for 48 hours

Next steps: If we get plaque we may try to get a high titer lysate  from our own sample, but we will most likely just use a classmate’s lsyate and start to work towards getting higher titer by first performing a plaque assay.