Rationale: The purpose of this lab is to perform serial dilutions.

Description of Procedures:

1. The workstation was cleaned using aseptic technique and an ethanol burner was lit to create an aseptic zone.
2. 90 ul of phage buffer was added to four tubes.
3. 10 ul of phage buffer lysate from the picked plaque was added to to the 10^-1 tube. 10 ul of the 10^-1 buffer was then added to the 10^-2 tube. This was done out to a dilution of 10^-4.
4. 10 ul of each tube was added to 0.5 ml of arthrobacter and allowed to sit for 10 minutes.
5. 10 ml of LB broth and 112.5 ul of CaCl2 were added to a tube. 12.5 ml of 2x TA were added to the tube.
6. 4.5 ml of the top agar solution was added to the 0.5 ml of arthrobacter and poured onto plates labeled LIP 10-29-18 PA 10^-1, LIP 10-29-18 PA 10^-2, LIP 10-29-18 PA 10^-3, and LIP 10-19-18 PA 10^-4. The rest of the top agar solution was poured onto a plate labeled LIP 10-29-18 control.
7. The plates were allowed to sit for 10 minutes and then inverted and stored in the incubator until the next lab.
8. The workstation was cleaned using aseptic technique and materials were properly stored and disposed of.

Observations:

• Bottom top agar had not set correctly on some plates.
• Bubbles seen on plates.

Interpretations/Next Steps:
The procedure was complete. The next step will be to make a webbed plate.