Rationale: Flooded the 10^0 plate, as well as calculated to web a plate by using more lysate.

Procedure:

• Created an aseptic zone to prevent contamination from other bacterium
• Obtained by plates from 10/22 and observed the results
• Flooded the 10^0 plate with 6mL PB and let sit for 48 hours
• Decided to run three plaque assays
  • 10^0 plate would be with 20μL lysate
  • 10^-1 would be 20μL 10^0 lysate + 80μL PB
  • 10^-2 would be 20μL 10^-1 dilution + 80μL PB
• Obtained a 50mL conical vial and added in 8mL LB Broth and 90μL CaCl2
• Added 10μL of 10^-1 and 10^-2 (Respectively) to 0.5mL arthrobacter
• Added 20μL 10^0 into 0.5mL arthrobacter
  • Let the arthrobacter+lysate sit for 10 minutes
• Added 10mL 2XTA to the conical vial and immediately pipetted 4.5mL into each arthrobacter+lysate vial and plated
• Allowed the plates to solidify and moved to incubation

Observations:

Contaminated TA control

10^-2 plate with markings on it to count the amount of plaques

10^-1 plate with a few plaques

Next Steps/Conclusion: Will be attending open lab on 10/26 to filter flooded plate and observe results of the plaque assays. If the plaque assays are positive, will calculate the titer of the lysate. If negative, will redo a plaque assay next lab.