10/24/18

Rational:

To run the samples from last lab through gel electrophoresis in order to determine whether there are arthrobacter phage present in the soil.

Procedure:

• Cleaned the lab desk with CiDecon and ethanol
• Weighted .72 g agarose powder
• Put 35 mL 1x TBE into a flask
• Added .72 g agarose powder
• Heated until the powder was dissolved and then it was cooled
• Added 1.7 ML EtBr and poured into the gel mold
• Then covered the gel with 1x TBE
• Added 5 ML of DNA ladder to a well
• Added dye to PM1 (primer mix 1)
• Added PM1, PM2, and PM3 to wells
• Turned on electrophoresis and waited 45 minutes

Observations:

• The picture of the gel electrophoresis had a white spot (possibly other DNA) covering the middle of the gel

Conclusion:

The spot in the middle of the image of the gel elctrophoresis prevented the results of the gel electrophoresis from being seen. As a result a new gel electrophoresis will be done next lab. A new soil sample will also be collected and filtered in case the results are negative.

Fig.4.F – This image shows the gel electrophoresis when it started. The dye around the wells is what overflowed out of the well when they were beig filled.

Fig.5.F – This image shows the results from the gel elctrophoresis. The white area seen in the middle may be DNA that leaked from a punctured well or from coming in contact with something else that has DNA on it.