Rationale: Re-calculate the titer strength of the lysate due to contamination of control plate and plaque assays

Procedure:

• First created an aseptic zone to prevent contamination of plates
• Obtained previous plates from incubation and observed contamination
• Obtained previous lysate from refrigeration as well as 4 petri dishes
• Created a 10^-1 dilution by adding 10μL 10^0 lysate to 90μL PB
• Created a 10^-2 dilution by adding μL 10^0 dilution to 90μL PB
• Obtained a 50mL conical vial and added in 10mL LB Broth and 112.5μL CaCl2 (Recipe was multiplied by 5 because a teammate used the same Lb Broth and TA)
• Added 10μL of lysate (Respectively) to 0.5mL Arthrobacter and let sit for 10 minutes
• Added 12.5mL 2XTA to the conical vial and immediately added 4.5mL to each arthrobacter + bacteriophage tube and plated
• Let the plates sit for 15 and incubated

Observations:

• 

  Contaminated Control Plate

  

  10^0 plate with no results; contamination prevalent

  

  Contaminated 10^-1 plate with no results

  

  Contaminated 10^-2 plate with no results

   

  Next Steps/Conclusion: The next steps would be to see if this current plaque assays turn out positive or not. If they do, calculating the titer of the plaque assay or flooding the plate would be the next course of action to create a high titer. If the plates turn out negative, then repeat the plaque assays.