Rationale: The purpose of this lab is to re-enrich soil C for further investigation.

Description of Procedures:

1. The workstation was cleaned using aseptic technique and an aseptic zone was created.
2. 2 ml of soil was added to a tube with 10 ml of LB Broth.
3. The soil was shaken for 10 minutes and then spun in the centrifuge for 10 minutes at 5,000 x g.
4. Supernatant was transferred to a tube and 0.5 ml of arthrobacter was added.
5. The tube will be shaken at room temperature until the next lab.
6. The workstation was cleaned using aseptic technique and materials were properly stored or disposed of.

Observations:

• Filtration process was not done due to no filters.

Interpretations/Next Steps:
The procedure was complete. The next step will be to filter and run a plaque assay.