Rationale: Wash newly collected soil in order to get both a direct and enriched sample isolation. In addition, start soil metadata experiments.

Process:

1. Wash table and set up aseptic zone
2. Added ~10 mL LB broth to ~2 mL soil
3. Shook and vortexed for 15 minutes
4. Centrifuged for 10 minutes
5. Filtered with syringe filter
   1. used ethanol to constantly clean tip
   2. ended with ~7 mL for enriched isolation and ~1 mL for direct isolation
6. Added 0.5 mL arthro to enriched isolation
7. Incubated enriched for 48 hours
8. Stored direct in fridge

Soil Metadata

• % H2O
  • mass of dry soil and weigh boat = 6.66 g
  • mass of wet soil and weigh boat = 6.77 g
  • mass of H2O = 0.11 g
  • mass of wet soil = 4.45 g
  • % H2O = 0.11 g / 4.45 g * 100% = 5.27%
• pH
  • added pinch of dirt to pH tube
  • filled with DI
  • shook for 10 seconds
  • held pH paper in for 45 seconds
  • pH = 6.0
• sand/silt/clay
  • added to falcon tube:
    • 4 mL soil
    • 8 mL  deionized water
    • 3 drops dispersion oil
  • shook for 45 seconds
  • let sit under hood for 48 hours

Next steps: Perform spot test to check for any plaques.