Rationale

Due to contamination that was present in the soil E enriched isolation made on 10/10/18, soil sample E must be washed and enriched again.

Procedure

• Established an aseptic zone.
• 2 mL of soil and 10 mL of LB Broth was added to a tube. The tube was vortexed for 10 minutes and then centrifuged at 5000g for 10 minutes.
• After centrifuging, the supernatant produced was filtered using a syringe filter and 0.5 mL of arthrobacter was added. The enriched isolation was placed into the incubator for 48 hours.
• In order to determine the amount of sand, silt, and clay present in the soil, the falcon tube was removed from the fume hood and the mL of sand, silt, and clay present were recorded.
• In order to determine the percent water in the soil sample, the soil sample was taken from the fume hood and was massed.

Results/Observations

The enriched isolation made on 10/10/18 possessed a slightly pink tint, mimicking a diluted color from the red bacteria that was present on the plates observed on 10/10/18.

Mass of the weigh boat: 5.327 g

Percent of water in the sample: 19.4%

Amount of sand: 4.5 mL

Amount of silt: 1.5 mL

Amount of clay: 1 mL

Percent sand: 60%

Percent silt: 26.6%

Percent clay: 13.3%

Conclusion/Next Steps

Next we will begin a new method of testing bacteriophage using our enriched isolation.