Rationale

Obtain rest of soil metadata to determine soil type, and percentage water. Start PCR process for enriched lysate from Lab Day 15 to determine if there is a present of plaque and whether or not if it can be tested through spot test/plaque assay in the future.

Detailed Procedure

1. Took 5 mL of unfiltered enriched lysate into a 15 mL vial and centrifuged it for 5 mins
2. Took 1 mL after centrifuge into a centrifuge tube. Labeled as S.J. Heated it up
3. Took 4 tubes that contained Taq polymerase+dNTP.
4. In first tube, PM1 (primer mixer) and 1 microliter of both DNA were added
5. In second tube, PM2 and 1 microliter of both DNA were added
6. In third tube, PM3 and 1 microliter of both DNA were added
7. In fourth tube, PM1, 1 microliter of positive control DNA, and 1 microliter of water were added.
8. All four tubes were labeled with group symbol and numbers and ran cycles

Soil Metadata Results

• plate after 48 hrs= 7.03
• percentage of water= 83.67%
• Sand= 42.42%
• Silt= 30.30%
• Clay= 27.2%

Conclusion

Soil E was determined to be a sand type soil. After the cycle for the 4 tubes are done and put into the fridge for next lab day, group 6 will be able to make gels to see and compare the DNA and determine which one of us have plaque (if present.) If it seems that there might be a hint that there is plaque, then spot test and plaque assay will be performed in the future.