Rationale: After contamination, the purpose of this lab is to create a plaque assay from the flooded lysate.

Description of Procedures:

1. The workstation was cleaned using aseptic technique and an aseptic zone was created.
2. 10 ul of the flooded lysate was added to 0.5 ml of arthrobacter and was allowed to sit for 10 minutes.
3. Top agar was made for two plates. 4 ml of LB broth and 45 ul of Calcium Chloride were added to a tube. 5 ml of 2x TA was then added and pipetted up and down to mix. 4.5 ml of the top agar solution was added to the 0.5 ml of arthrobacter and poured onto a plate labeled LIP 10-17-10 PA. The rest of the top agar was added to a control plate labeled LIP 10-17-18 control.
4. The plates were allowed to sit for 10 minutes to solidify and then inverted and stored in the incubator until the next lab.
5. The workstation was cleaned using aseptic technique and materials were properly stored and disposed of.

Observations:

• Small bubbles were seen on both plates, very few.

Interpretations/Next Steps:
The procedure was complete. The next step will be to check for plaques. If plaques are found, then serial dilutions will be performed. If no plaques are found, the purification process will need to be performed again.

Contaminated Plaque Assay and Control: