10/12/18

Rational:

To record results for the soil metadata and to perform a plaque assay on soil E to test for the presence of arthrobacter phage in the soil sample.

Procedure:

• Cleaned the lab desk with CiDecon and ethanol
• Filtered the enriched lysate to get FS lysate
• Put 10 ML FS lysate into .5 mL arthro
• Put 2 mL LB broth into soil E TA and control
• Added 22.5 ML CaCl2 1 M to soil E TA and control
• Added .5 arthro and lysate to soil E TA
• Added 2.5 mL TA to the control and poured on the plate (used maltose agar plate)
• Added 2.5 mL TA to the soil E TA and poured on the plate
• Waited 10 min and then put the plates in the incubator at 26 C at 3:15
• Weighed dry soilfrom last lab and measured the amount of sand silt and clay present in the soil sample

Observations:

• Dry soil and weigh boat-
• Dry soil- 4.02 g
• Percent water- 3.8%
• Sand- 2.5 mL
  • Percent sand- 25%
• Silt- 4.5 mL
  • Percent silt- 45%
• Clay- 3 mL
  • Percent clay- 30%
• Soil type- Clay Loam

Conclusion:

The soil texture for soil E was the same as soil B (clay loam). The percent water for soil E is also very similar to soil B and D, but lower than the percent water found in soil C. Next lab I will check for plaque on my plate and if there are plaque then I will start purification. If there is no plaque or if there is contamination I will do the technique that everyone else who has not found a plaque yet will be doing.

Fig.5.E – This image shows the plate for soil E plaque assay. The yellow circles indicate where bubbles in the top agar ar located.

Fig.6.E – Show the control plate for soil E plaque assay. The yellow circles show the location of bubbles in the control plate’s top agar.

Shows the tube containing a sample of soil E which show the amount of sand, silt, and clay in soil E.