10/10/18

Rational:

To get a new soil sample, collect soil metadata and filter it so that a new plaque assay can be done.

Procedure:

• Collected a new soil sample 2 ft from the base of a Post Oak
• Cleaned the lab desk with CiDecon and ethanol
• Put 2 mL of soil into a tube and added 10 mL LB broth
• Shook the tube for 15 min then centrifuged it at 3000 G for 10 min
• Weighed and empty weigh boat and then weighed it with some of the soil sample in it
• Placed the weigh boat under the hood
• Put 10 mL of the soil sample into a falcon tube
• Added 20 mL DI water and then added 3 drops of soil dispersion
• Shook the tube for 30 sec and then place the tube under the hood
• Put some of the soil sample into a pH vial and added DI water
• Shook the vial for 10 sec and then checked the pH
• Filtered the soil sample that was in the centrifuge and added .5 mL arthro
• Put the enriched lysate into the shaking incubator at 26 C at 4:00

Observations:

• The leaves on the Post Oak were slightly brown and seemed to have been eaten by something
• Tree height- 1,066.8 cm
• Tree trunk circumference- 106.7 cm
• Canopy width- 457.2 cm
• Empty weigh boat- 1.98 g
• Weigh boat with soil sample- 6.93 g
• Wet soil weight- 4.95 g
• pH of soil E- 6.5

Conclusion:

The second plaque assay for soil D showed no plaque so a new soil sample was collected. Soil metadata was also collected and the pH of the sample was 6.5 which is slightly closer to neutral (less acidic) than the other samples. The sample was also filtered and enriched for next lab. Next lab I will do a new plaque assay for the new soil sample and record the results for the soil metadata

Fig.1.E – This image shows the tree that the new soil sample was collected from.

Fig.2.E – Shows a healthy looking leaf from the Post Oak that the soil sample was collected from.

Fig.3.E – The leaves in this image have been eaten by something especially at the bottom of the leaves.

Fig.4.E – Shows the pH of this soil sample is 6.5.