October 11

Soil D Plaque Assay 2

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10/8/18

Rational:

To do another plaque assay to look for the presence of arthrobacter phage in a plate that is not contaminated.

Procedure:

  • Cleaned lab desk with CiDecon and ethanol
  • Added 10 ML FS lysate into .5 mL arthro
  • Put 2 mL LB broth into the TA mixture and control
  • Added 22.5 CaCl2 1M to TA mixture and control
  • Poured .5 ML arthro and lysate into TA mixture
  • Put 2 mL TA into the control and poured on the plate
  • Put 2.5 mL TA into the TA mixture and poured on the plate
  • Waited for 10 min and then put plates in the incubator at 26 C at 3:30-2:30 10/10

Conclusion:

The first plaque assay for soil D was possibly contaminated s the control showed signs of contamination. As a result a new plaque assay was done. Next lab I will check the plate for plaque and if there is plaque then I will start the next step (purification). If there is contamination or no plaque then I will find a new soil sample and filter and collect metadata on it.

Fig.10.D – Shows the plaque assay from 10/5. The clearings show where there were bubbles in the TA and are not plaque.

Fig.11.D – Shows the control plate. The white spots covering the plate indicate that it was contaminated.


Posted October 11, 2018 by emily_balint1 in category Emily Balint

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