October
11
Soil D Plaque Assay 2
10/8/18
Rational:
To do another plaque assay to look for the presence of arthrobacter phage in a plate that is not contaminated.
Procedure:
- Cleaned lab desk with CiDecon and ethanol
- Added 10 ML FS lysate into .5 mL arthro
- Put 2 mL LB broth into the TA mixture and control
- Added 22.5 CaCl2 1M to TA mixture and control
- Poured .5 ML arthro and lysate into TA mixture
- Put 2 mL TA into the control and poured on the plate
- Put 2.5 mL TA into the TA mixture and poured on the plate
- Waited for 10 min and then put plates in the incubator at 26 C at 3:30-2:30 10/10
Conclusion:
The first plaque assay for soil D was possibly contaminated s the control showed signs of contamination. As a result a new plaque assay was done. Next lab I will check the plate for plaque and if there is plaque then I will start the next step (purification). If there is contamination or no plaque then I will find a new soil sample and filter and collect metadata on it.
Fig.10.D – Shows the plaque assay from 10/5. The clearings show where there were bubbles in the TA and are not plaque.
Fig.11.D – Shows the control plate. The white spots covering the plate indicate that it was contaminated.