Rationale: the arthro from the last class was probably contaminated because the positive controls did not yield any plaques. The results from the previous spot test are inconclusive so the experiment had to be repeated

Invalid spot test results from previous class

Process:

1. Set up aseptic system
2. Made LB agar media for 1 control plate and 1 sample plate
   1. We only had 400 µL of arthro available so 5 samples were spotted on the sample plate

   2. Reagents	Control	Sample
      LB Broth	2.1 mL	4.2 mL
      2x TA	2.5 mL	5 mL
      CaCl2	23 µL	45 µL
      arthro	NA	400 µL

3. poured 5 mL of LB agar media into each plate and let sit ~5 min
4. pipetted 5 µL of direct (RSM), direct (LCG), enriched (RSM), enriched (LCG), and phage buffer (negative control) into designated areas of sample plate and let sit ~10 min
5. incubated for 48 hours

Next steps: If the spot test is positive, perform a plaque assay to check for plaques again. If the spot test, is negative, dig more dirt!