Rationale

Redo plaque assay due to possible contamination of Arthro from lab day 9. Other group members will redo spot test.

Detailed Procedure

1. Took 10 microliters of filtered enriched lysate and added to 400 microliters of Athro. Sat for 15 mins
2. Took 4.2 mL LB Broth, 45 microliters of 1M CaCl2, and 5 mL of 2X TA into a 50 mL vial.
3. Took half of solution from step 2 and poured into control plate
4. Took other half and added solution from step 1 and poured into plaque assay plate
5. Both plates sat for 15 mins and was inverted into the incubator.

Conclusions+Observations/Results/Next Steps

Control plates from group members’ spot test and plaque assay were both contaminated, but showed negative results. There seems to be contamination of Arthro from class discussion. When pouring control plate, there was a bubble on the plate that did not pop. Next steps are to wait for the results. If no plaque are present for both spot test and plaque assay, then new soil will have to be found.