Rationale

Perform both spot test and plaque assay from Soil C without any trace of contamination. Record results of soil metadata from last lab day to determine the type of soil Soil C is. Two members from group 6 did spot test while one did plaque assay. Each test had an individual control plate.

Detailed Procedure

1. Syringe filtered enriched lysate.
2. Took 10 microliters of filtered enriched lysate and added to 0.5 mL Arthro. Sat for 15 mins.
3. During 15 mins, took 4.0 mL LB Broth, 45 microliters of 1M CaCl2, and 5.0 mL of 2X TA into separate 50 mL vial. Mixed through pipetting.
4. Used sterile pipette and took half of solution from step 3 into another separate 15 mL vial. Labeled as control.
5. After 15 mins were done, took solution from step 2 and mixed through pipetting into previous 50 mL vial.
6. Took both vials into water bath and waited for plate to be ready.
7. Labeled plates as “Plaque Assay” and Plaque Assay Control.”
8. Took control vial and poured into control plate and same with other plate+vial.
9. Waited 15 mins to solidify.
10. Inverted plates into incubator.

Conclusions/Results/Observations

• Clay= 75%
• Silt= 18.75%
• Sand= 6.25%
• Soil C is clay based sand

Due to more experience, there were no bubble on both plates and was able to prevent possible contamination (accidental spills, not being in aseptic zone.) In the next lab day, future steps would be to check for any signs of plaque. Since within the group, both spot test and plaque assay was performed, so if no plagues were found, then a new soil will be tested next.