September
20
Lab Day 7: Results
Rationale
Check to see if any plaque remains. If not, why/how does our results mean to the group question. Find new soil. Control plate from Lab day 6 was contaminated, but showed negative results from plaque assay plate. Other group members did spt test and had negative results as well.
Detailed Procedure
- Dug up new soil from a red oak in between the BSB and business building.
- Filled 15 mL vial up with soil up to 2 mL mark
- Filled bag with soil
- Began soil metadata by weighing plate and soil
Results
- weight of plate= 2.34 g
- weight of plate + soil= 6.68 g
Group question
- Lathan checked a purified lysate by doing a plaque assay (10 microliters lysate) of a 10^-3 lysate. He wanted 14 plaques. How many microliters of Lathan’s lysate?
Conclusion
New soil’s metadata must be done by next lab day to determine which type of soil we collected. Soil enrichment will also be done by next lab day.