Rationale: Analyze the hard data of the tape measure proteins, and to finish final abstract.

Procedure: DNA Master, google slides, Phagesdb, and google docs were used to gather information from the analyzation of tape measure proteins. Jmol was used to help construct models for the proteins.

Results:

Clusters

• AY 6 phages
  • Alone AY has ⅖ UUG start
• FE 3 phages (we have 2)
  • Idaho UUG start 1827bP. GC content 63.6
  • Corgi and Noely 1672bP average. GC content 67.9
• Singleton 7 (we have 1 all others were drafts/not sequenced)
  • UUG start
• AM
  • 4478bP
  • Pham 45114
  • Start AUG

NapoleonB GUG

NapoleonB AUG

NapoleonB UUG

Arcadia AUG

Arcadia GUG

Arcadia UUG

Conclusions: AM cluster phages tape measure protein share lots of unique features, but yet when start sites for these phages were altered, the structures were different. Six models are represented in the results, both are from the AM cluster. AUG and GUG are very similar, but compared to UUG, you can see differences.

Future work: Literature is needed to support why different structure and how this structure may have an affect on the proteins tail assembly, tail length determination, connection of capsid and distal tail regions, or genome delivery upon infection.