Rationale:

To understand more about phage, the class was divided into several small groups to generate research questions that utilize bioinformatic tools to learn more about questions about NapoleonB and/or related phages.

Materials:

• Laptop
• Canvas Bio-Lab info page
• DNA Master
• NCBI & Phagesdb Database

Procedure & Results:

The sequence was not backed by transposon finder software(IS finder), the project was then discussed with coaches and the remaining possibility for a conserved sequence within a cluster might be a recognition site for gene transcription such as a promoter.

The sequences were all found in front of the gene of the same pham in the AM cluster, which further supports the hypothesis.

The Next Step:

The next step would be to try to find supporting evidence that backs up the new hypothesis.