Rationale:

The purpose of today’s lab was to create an abstract for the powerpoint presentation as well as look at lysin A catalytic regions.

Tools:

• JMOL
• Word
• NCBI

Procedure:

• Drafted the abstract for the QTM
• Used JMOL to look at Lysin A structures to determine the possible catalytic region
• Researched the PGRP superfamily

Results:

The Written abstract

The Folded Lysin A Protein

Conclusions:

The lysin A protein has the same structurally conserved catalytic region as the annotated “endolysin” in NapoleonB. Both proteins contain the same beta sheet structure, while the lysin A is missing the alpha helices at the end of the molecule that NapoleonB’s endolysin possesses.

Next Steps:

Fold the PGRP proteins and determine catalytic region as well as any other lysin variation we find.