Rationale: 

Start Codons for AQ Cluster were finished, and average amounts of each start codon and GC content for each phage were calculated and noted.  

Tools: 

• PhagesDB 
• DNA Master 
• Excel  

Procedure:  

1. FastA file of each of the phages in AQ Cluster were downloaded.  
2. The FastA file was opened in DNA Master.  
3. Start codons for each of the phages were noted onto Excel.  
4. Averages of each start codon for the phages were calculated.  
5. GC content for each of the phage was obtained from PhagesDB.  

Results:  

Example of data collection of start codon for phage Anasi:  

Conclusion:  

It was observed and reported in an article that the start codon ATG, GTG, and TTG were respectively 45%, 45%, and 7%. However when looking at the data that was collected, the percent of ATG was much higher, usually about 80%, in each phage, and the GTG and TTG start codons, which was about 5-10% each. 

Future Work:  

Start codons for AU cluster will be collected, and a phylogenetic tree will be constructed to see if there any way the phages could be sub-clustered.