Presentation Outline and Continued Research 4/15/19

Rationale

The rationale behind today in lab was to create a project outline to help guide the rest of our research and to help us begin to prepare for our final presentations. In addition, when we were done with our presentation outline, we continued working on sequencing and protein folding in order to collect the necessary data.

Tools/Procedure

1. Presentation outline was submitted
2. MEME Motif was used to find repeating motifs in the TMPs of selected proteins from each usable cluster

Results

The results above were generated using the MEME software to find motifs. The logo above represents a motif that was found in all selected TMPs except one, suggesting an area of conservation. When all of the TMPs have been folded, we will see if we can use the protein folding information to support this motif being conserved structurally as well.

Conclusion

While there is not enough data to make extensive conclusions yet, our data so far suggests that while there are significant differences among TMPs outside of their clusters, there do appear to be at least some similarities, which answers our guiding question.

Future Plans

In the future, we will continue to look for similarities and difference in the amino acid while continuing our folding of proteins to try to figure out if there are key conserved regions in TMPs. I will compare all 43 selected TMPs to ensure that the above-identified motif is as universal as it appears.