Rationale: After talking to Dr. Pope at the phage emporium I decided the best move is to look at modules containing genes that originate in bacteria to find more superclusters. By finding more superclusters I can compare against the one I’ve already found to make the claim that these are a thing.

Tools: PhagesDB, Phamerator

Procedure:

1. I started by searching through phams in phagesDB that are called as cas4 exonucleases.

2. I looked at the phamerator maps of multiple clusters containing a cas4 exonuclease and tried to find patterns in the gene synteny.

3. Discovered another instance of what might be a supercluster that contained a module of DNA primase followed by a DNA polymerase.

Results:

Clusters found to contain similar synteny and the 45806 primase module:

• AZ
• BB
• BB1
• BB2
• BJ
• BL
• EB
• EH

Conclusions and Future Work: This method of discovering new superclusters seems to work quite well I’m going to continue looking for more superclusters so I can compare them against each other.