Purpose: The purpose of this lab was to begin outlining the presentation for the research project and the final presentation in May.

Tools/Procedures:

Tools:

• Google Drive
• PubMed/NCBI
• PhagesDB

Procedures:

1. The data from the last lab was analyzed and discussed to determine the next step in the project.
2. It was decided that a literature search would need to be done to learn more about the %GC in host and phage genome, and the relationship between their GC content.
3. Articles were found discussing the phage and hos GC content.
4. A project outline was created for the presentation.
5. An abstract was started, and a background information section was also begun. The results are not finished, but the current findings were compiled. The conclusion was also begun.

Results:
An outline for the project was created, with elements such as the abstract, background information, and conclusion. The articles found showed that in most bacteriophage there is a correlation between their %GC and that of their host. It was also found that the longer the phage genome was, the lower the %GC. It was also found that the average %GC of Arthrobacter sp. ATCC 21022 is 63.41%.

Conclusions:
In conclusion, it was determined that more literature needed to be read to try and determine the reason that the AM phage %GC is so much lower than that of its host. An outline was created for the final presentation.

Future Work:
Future work will include learning more about the GC correlation of phage and their host species through literature searches. Also, more work will need to be done for the final presentation.