Title: Transposon Searching

Date: 8 April 2019

Rationale: We will continue to search for a purpose or additional information that can define the 45-bp sequence (with 6-bp palindromic flanks)

Procedure: Upon BLASTing the sequence again on PhagesDB, it was found that 11 out of 14 phages contain the sequence, with 2 phages, NapoleonB and Kardesai, containing 2 copies of the sequence. The phamerator maps were inspected and more research was done to find literature that could support the finding being a promoter or transposon, or otherwise.

Results/Observations: All phages, except for the 2 mentioned above, that contained the sequence had a gene sequence consensus of a gene in pham #37812 followed by pham #1350. However, the 2 phages that contained a copy of the sequence had a gene in pham #37812 followed by a gene in pham #620. It is worth noting that only NapoleonB and Kardesai have copies of this gene phamily.

Conclusions/Next Steps: All of the sequences will be inspected to look at the flanking regions as well as searches conducted on the internet to find programs that may be able to predict transposons or promoters.