April
12
Independent Research Project 4/8/19
Rationale
Today we will continue working on our independent research projects. We aim to gather more data on NapoleonB and other AM cluster phages.
Procedure
- The annotations created by the class were uploaded onto DNA Master.
- The %GC and %GC3 were calculated for each gene in Napoleon and the results were recorded into an excel sheet.
- The genes and their %GC and %GC3 values were sorted into two categories of whether the gene was AM specific or non-AM specific.
- The average %GC and %GC3 values were calculated for each group created.
- The process was repeated for a non-AM cluster phage.
- Average %GC was also collected for each specific type of Arthrobacter phage.
Results
Shown below are the recorded average %GC for each cluster of Arthrobacter phage.
Conclusion/Next Steps
Next, we will perform a t-test with our results and move onto data collection for other Arthrobacter phages.