April
12
April 8th + 10th- labs
- APRIL 8TH 2019
- OBJECTIVE:
- Find more primary literature to read in order to deepen our understanding of the project being worked on
- PROCEDURE:
- NCBI was used to search for sources
- Annotations were then created for those sources
- RESULTS:
- Sources listed below:
- https://academic.oup.com/femsre/article/30/3/321/546048
- Weigel, Christoph, and Harald Seitz. “Bacteriophage Replication Modules.” FEMS Microbiology Reviews 30, no. 3 (May 1, 2006): 321–81. https://doi.org/10.1111/j.1574-6976.2006.00015.x.
- https://www.sciencedirect.com/science/article/pii/S1084952117306055
- Hernandez, Alfredo J., and Charles C. Richardson. “Gp2.5, the Multifunctional Bacteriophage T7 Single-Stranded DNA Binding Protein.” Seminars in Cell & Developmental Biology, SI: Human dendritic cells, 86 (February 1, 2019): 92–101. https://doi.org/10.1016/j.semcdb.2018.03.018.
- https://doi.org/10.1016/S0378-1097(99)00068-3
- Hamann, Christian, Jörg Hegemann, and Armin Hildebrandt. “Detection of Polycyclic Aromatic Hydrocarbon Degradation Genes in Different Soil Bacteria by Polymerase Chain Reaction and DNA Hybridization.” FEMS Microbiology Letters 173, no. 1 (April 1, 1999): 255–63. https://doi.org/10.1111/j.1574-6968.1999.tb13510.x.
- https://academic.oup.com/mbe/article/23/9/1688/1014265
- Filée, Jonathan, Eric Bapteste, Edward Susko, and H. M. Krisch. “A Selective Barrier to Horizontal Gene Transfer in the T4-Type Bacteriophages That Has Preserved a Core Genome with the Viral Replication and Structural Genes.” Molecular Biology and Evolution 23, no. 9 (September 1, 2006): 1688–96. https://doi.org/10.1093/molbev/msl036.
- https://www.sciencedirect.com/science/article/pii/S0042682218300588?via%3Dihub
- Jalasvuori, Matti, and Katariina Koskinen. “Extending the Hosts of Tectiviridae into Four Additional Genera of Gram-Positive Bacteria and More Diverse Bacillus Species.” Virology 518 (May 1, 2018): 136–42. https://doi.org/10.1016/j.virol.2018.02.014.
- CONCLSUION:
- Articles were read, and annotations were made
- FUTURE STEPS:
- Continue researching gene functions
- –APRIL 10TH 2019
- OBJECTIVE:
- Create a document with all the sequences that need to be run through data bases
- PROCEDURE:
- A document was created and the gene for the terminase of a phage belonging to each of the following clusters was created:
- AU1 AU2 AU3 AW BI1(bing) BI2 BI3 BI4 CC DJ EL
- RESULT:
- N/A
- CONCLUSION:
- Document was created
- FUTURE STEPS:
- Finish adding codes for each terminase protein for a phage of each cluster