Rationale: Find date for independent research projects which support the scientific question

Materials:

• DNA Master
• PhagesDB
• Google SpreadSheet

Procedure:

• Opened DNA Master and opened FASTA files of phages in AM cluster
• Recorded each start codon for each gene
• Determined the preference percentage of each start codon (ATG, TTG, GTG)

Observations:

NapoleonB with the preferred percentages of each start codon

Mudcat with the preferred start codon of each gene

Results/Next Steps:

• When observing the preferred start codons, there was a pattern in the last four genes of the phages in the AM cluster. They all preferred a GTG-GTG-ATG-ATG for their last 4 genes, which may show that they code for similar genes. This discovery can help other scientists help characterize and cluster their phage earlier in research. Will continue to find the preferred start codons of the AM cluster as well as the AR cluster