Rationale: Continue to gather data to answer the scientific question regarding preferred start codons and the relationships that can be drawn between clusters and phages within clusters

Materials:

• DNA Master
• Google SpreadSheet
• PhagesDB

Procedure:

• Loaded up the FASTA files from PhagesDB of the AR phage and annotated them in DNA Master
• Recorded the start codons of each gene and calculated the percentage of each start codon (ATG, GTG, TTG)

Observations:

• 

  The preferred start codons of phage KBurrousTX

  

  The preferred start codons of phage Linus

   

Conclusion/Next Steps: Similar to the AM cluster, there were patterns within the AR cluster which is unique to the phages found within the cluster. In the examples of phage KBurrousTX and Linus, the last five genes of the phages preferred an ATG-GTG-ATG-ATG-ATG pattern which was found throughout all the phages in the AR cluster. The next steps would be to calculate the preferred percentage of the start codons and observe if there is a correlation between clusters or phages in the clusters.