Rationale:

To continue working on our IRP by looking into the NMN pathway and NMT related proteins in other organisms and phages, we will also run MSA’s in order to determine phylogenetic relationships.

Procedure:

1. I conducted further background research into NMT and NMN.
2. We looked at other phages in the gene 93 pham.
3. We investigated the differences in NMT, NRT, and NRTransferase, using both literature and HHPred.
4. We ran multiple sequence alignments.

Data/Results:

We found that there may be apparent errors in either the pham organization or gene calling of other phages in regards to NMT, NRT, NRTransferase. We also found some relations between the various phages and bacteria compared via MSA but some error may have been involved, but it does appear that there are relations.

Conclusions/Next Steps:

We can conclude that there are relations between gene 93 in NapoleonB and similar genes in other organisms/phages, which will ultimately allow us to find phylogenetic relationships and help us to determine the origin of gene 93. We can also conclude that something weird/incorrect is happening with the pham organization and the gene calling of other phages. Our next step will be to continue investigating phylogenetic relationships and running MSAs, while also looking at protein structures in order to provide evidence/support.