Data Gathering

Date: 4-8-19

• Rationale
  • The rational for this lab is to continue gathering data to use for our independent research project.
• Procedure
  1. PhagesDB was used to find the entire nucleotide sequence for phages in the AM cluster.
  2. SplitsTree was used to produce a phylogenetic tree for the AM cluster phages.
  3. Start codon preferences for each phage were calculated and recorded on the google sheets page used in previous labs.
• Results
• 
  • Above is a screenshot of a phylogenetic tree including every AM cluster phage. This was made using whole genome alignment between the phages.
• 

  NapoleonB’s preferred percentages of each start codon

  

  Mudcat’s preferred start codon of each gene

• Future Plans
  • The next step is to continue gathering this data before attempting to further subcluster phages based on start codon preference.