Data Gathering – AQ and AR Clusters

Date: 4-10-19

• Rationale
  • The rational for this lab is to continue gathering data to use for our independent research project.
• Procedure
  1. PhagesDB was used to find the entire nucleotide sequence for phages in the AQ and AR clusters.
  2. SplitsTree was used to produce phylogenetic trees for the AQ and AR cluster phages.
  3. Start codon preferences for each phage were calculated and recorded on the google sheets page used in previous labs.
• Results
• 
  • Above is a screenshot of a phylogenetic tree including every AR cluster phage. This was made using whole genome alignment between the phages.
• 
  • Above is a screenshot of a phylogenetic tree including every AQ cluster phage. This was made using whole genome alignment between the phages.
• 

  The preferred start codons of phage KBurrousTX

  

  The preferred start codons of phage Linus

• Future Plans
  • The next step is to continue gathering this data before attempting to further subcluster phages based on start codon preference.