Rationale:

Analyze endolysins and holins to collect more data to create a presentation to present at SEA-PHAGES symposium.

Procedure:

1. Printed out and analyzed RaptorX structures created previously.
2. Outlined SEA-PHAGES symposium presentation.
3. Used Blender to model holin’s hole formation.

Results:

The following images shows the endolysins and holins structure predicted with RaptorX.

The following image shows the unfinished holin creating holes in the plasma membrane model.

Conclusion:

Compared with Multiple Sequence Alignment and phylogenetic tree results, the predicted protein structure visualized with RaptorX revealed that there are although sequences were quite similar, the structures varied.

Future Work:

Finish creating presentation and present poster and presentation at SEA-PHAGES symposium.