April 11

Major tail protein structural analysis 4/10

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Rationale: Begin major tail protein structural analysis and to finish hard data for both minor tail proteins and tail proteins.

Procedure: DNA Master, PhagesDB, Google Sheets, and SWISS-MODEL Workspace were used to in major tail protein structural analysis. Obtained amino acid sequence from PhagesDB and uploaded the sequence to SWISS-MODEL Workspace and the structures were produced. Analysis of sturures were performed and lots of differences were found.

Results:  

Heisenberger 15 Major tail proteins both with the start AUG

some of the tail proteins hard data were obtained but not all. One of these tail proteins had a 399bP length, and that is really small for a tail protein. More research on this will have to be done to see why this particular protein is extremely small compared to the others.

Conclusions: When the Heisenberger 15 amino acid sequence was placed into SWISS-MODEL Workspace, these were 2 of the 3 structures produced. Two of the structures were the same but as you can see from the results, two are different, and they both have the same amino acid with the preferred starts. More work is being done to analysis the different structures of the major tail protein. Changing the starts of some tail proteins had no effect to the overall structures, but some proteins had different structures just from the preferred start site.

Future work: Begin tail protein and minor tail protein structural analysis. Finish both tail proteins and minor tail proteins hard data by using PhagesDB and DNA Master.


Posted April 11, 2019 by michael_lum1 in category Michael Lum

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