April 10 2019 Independent Research Continued
Purpose: The purpose of this lab is to continue work on the final project, collecting and analyzing data.
Tools/Procedures:
Tools:
- DNA Master
- PhagesDB
- PhageNotes
Procedures:
- The genes of NapoleonB were analyzed to determine which genes were specific to the AM cluster, and which were not.
- Statistical analyses were performed on the data found in the last lab.
- It was found that the AM-specific genes had a statistically significant lower %GC than the non-AM genes.
- No significant difference was found for the %GC3 of the AM and non-AM cluster genes.
- It was also determined that the arthrobacter AM cluster phages have a significantly lower %GC than all non-AM arthrobacter phages.
Results:
The analyses of the data collected show that AM cluster phages have %GC that are lower than the average arthrobacter phage %GC. While all of the genes in NapoleonB have a lower average %GC, the AM-specific genes have an even lower average than the non-AM specific genes.
| %GC | Difference | Sample Diff. | Std. Err. | DF | T-Stat | P-value |
| μ1 – μ2 | 1.89 | 0.66921299 | 95.842177 | 2.824213 | 0.0029 |
Conclusions:
In conclusion, it was found that AM cluster genes have lower %GC than most other arthrobacter phage clusters.
Future Work:
Future work will include gathering more data about AM cluster genes, and trying to determine why they have a lower %GC than other genes. This could include looking at genes not found in AM phage, as well as looking at what makes AM phages different from the other arthrobacter clusters.