Purpose: The purpose of this lab is to collect sources for the independent project, and to continue researching the project question.

Tools/Procedures:

Tools

• DNA Master
• PhagesDB
• Google Sheets

Procedures:

1. Sources were found for the project and citations were made for each of them.
2. %GC for all arthrobacter phage were compared and the average was found.
3. %GC and GC3 were found for all of NapoleonB’s genes., an AM cluster phage.
4. %GC and GC3 were found for all of Sonali’s genes, an FG cluster phage.
5. Averages and standard deviations were found for the above information.
6. %GC was compared for all AM specific genes to the %GC of non-AM specific genes.
7. Research was saved.

Results:
In this lab, data was collected for the %GC of genes in an AM cluster phage, the arthrobacter cluster with the lowest %GC, and for an FG cluster phage, the arthrobacter cluster with the highest %GC. The data will be analyzed in a future lab.
It was found that Arthrobacter phage have an average %GC of 59.7%, while AM phage have an average 0f 45.2%.
For NapoleonB, it was found that the phage had an average %GC for its genes of 44.9%, and GC3 of 43.97%
For Sonali, it was found that the phage had an average %GC for its genes of 67.7%, and GC of 89.6%

Conclusions: 
In conclusion, this lab was used to begin data collection on %GC and GC3 of different clusters and different Arthrobacter phage. Overall, it was found that the %GC in Arthrobacter clusters has a high standard deviation, meaning it has a wide range. However, the standard deviation for AM compared to other Arthrobacter clusters, had an even higher standard deviation. It was also found that the GC3 of Sonali’s genes differed sigificantly from the %GC of the genes, and from the average %GC.

Future Work:
Future work will include collecting more data for AM and non AM cluster phages, concerning their %GC. Also, in the future, the data collected will be analyzed, to see if there is a significant difference, and why.