March 22

Individual Project 3/20/19

Rationale: Begin brainstorming ideas for individual project

Process:

  1. ideas:
    • investigate different plaque morphologies
    • investigate host range
    • create program to analyze pharmerator maps and analyze information in a new way

Conclusion:

  • still need an idea because all of the above had different problems of some sort

Next Step:

solidify an idea

March 22

Class Poster Editing 3/22/19

Rationale: Work on parts of poster as a class to compete a final version

Process:

  1. began working in graphic design group to design small images to put onto the poster
    1. Main contributions:
      1. created the worst design that was too small
      2. found the exact code for the color used on the poster
  2. Joined Ram’s group to help create data figures for in silico results
    1. main contributions:
      1. created a c++ program that parsed through a text block generated from a pharmerator image to extract different NapoleonB information

Results:

code:

#include <iostream>
#include <fstream>
#include <cctype>
#include <string>

using namespace std;

struct Entry {
    string id;
    int code;
    int num;
};


int main() {
     ifstream in;


     in.open("data.txt");


     int count = 0;
     int ecap = 100;
     Entry *entries = new Entry[ecap];

     string save[5000];
     int scnt = 0;
     char name[40];
     char num[10];
     int nc = 0;
     char a[40];
     char *b;

     while ( in >> a ) {
         save[scnt] = a;
         char *da = a;

         while (*da != '\0')
         {
             if (*da == 'i')
             {
                 da++;
                 if (*da == 'd') {
                     da++;

                     if (*da == '=')
                     {
                         char *dname = name;
                         da += 2;

                         while (*da != '"') {
                             *dname = *da;
                             dname++;
                             da++;
                         }

                         *dname = '\0';
                         //cout << name << endl;
                         entries[count].id = name;
                         count++;
                         //cout << entries[count].id << endl;
                     }
                 }
             }
             da++;
         }

         if ( a[0] == '(' )
         {
             //cout << save[scnt -1] << endl;

             char *na = a;
             char *dnum = num;

             na++;

             while ( *na != ')' )
             {
                 *dnum = *na;
                 dnum++;
                 na++;
             }
             *dnum = '\0';

             entries[nc].num = atoi( num );

             string codeLine = save[scnt-1];

             string codeS = codeLine.substr(codeLine.find(">") + 1);


             entries[nc].code = stoi( codeS );
             //cout << entries[nc].code << " " << nc << endl;
             nc++;

         }

         scnt++;

     }

     in.close();

     //bubble sort

    Entry temp;
    bool madeAswap;

    do
    {
        madeAswap = false;
        for ( int i = 0; i < 100; i++ )
        {
            if ( entries[i].num > entries[i + 1].num )
            {
                temp = entries[i];
                entries[i] = entries[i+1];
                entries[i+1]= temp;
                madeAswap = true;
            }
        }
    } while ( madeAswap );

    for ( int i = 0; i < 100; i++ )
    {
        cout << entries[i].num << ","
             << entries[i].id << ","
             << entries[i].code << endl;
    }





     return 0;
}

Generated List:
1,NAPOLEONB_DRAFT_30,8973
1,NAPOLEONB_DRAFT_46,2172
2,NAPOLEONB_DRAFT_18,17808
2,NAPOLEONB_DRAFT_28,620
2,NAPOLEONB_DRAFT_82,9032
3,NAPOLEONB_DRAFT_29,9160
3,NAPOLEONB_DRAFT_80,6235
4,NAPOLEONB_DRAFT_35,5503
4,NAPOLEONB_DRAFT_64,23957
4,NAPOLEONB_DRAFT_66,14746
6,NAPOLEONB_DRAFT_43,14427
6,NAPOLEONB_DRAFT_96,5481
7,NAPOLEONB_DRAFT_94,16425
8,NAPOLEONB_DRAFT_78,7010
8,NAPOLEONB_DRAFT_88,16636
9,NAPOLEONB_DRAFT_90,2220
10,NAPOLEONB_DRAFT_17,15199
10,NAPOLEONB_DRAFT_89,17851
11,NAPOLEONB_DRAFT_27,20203
11,NAPOLEONB_DRAFT_40,2745
11,NAPOLEONB_DRAFT_65,15086
12,NAPOLEONB_DRAFT_55,3201
13,NAPOLEONB_DRAFT_63,16469
14,NAPOLEONB_DRAFT_21,1334
14,NAPOLEONB_DRAFT_22,5598
14,NAPOLEONB_DRAFT_31,1350
14,NAPOLEONB_DRAFT_32,12456
14,NAPOLEONB_DRAFT_33,7647
14,NAPOLEONB_DRAFT_34,13454
14,NAPOLEONB_DRAFT_36,15534
14,NAPOLEONB_DRAFT_4,24501
14,NAPOLEONB_DRAFT_41,4750
14,NAPOLEONB_DRAFT_45,15236
14,NAPOLEONB_DRAFT_47,9175
14,NAPOLEONB_DRAFT_48,21289
14,NAPOLEONB_DRAFT_49,8333
14,NAPOLEONB_DRAFT_50,4594
14,NAPOLEONB_DRAFT_51,5790
14,NAPOLEONB_DRAFT_52,2845
14,NAPOLEONB_DRAFT_57,11576
14,NAPOLEONB_DRAFT_59,11096
14,NAPOLEONB_DRAFT_6,21245
14,NAPOLEONB_DRAFT_60,15626
14,NAPOLEONB_DRAFT_67,6336
14,NAPOLEONB_DRAFT_68,7770
14,NAPOLEONB_DRAFT_70,9736
14,NAPOLEONB_DRAFT_72,9734
14,NAPOLEONB_DRAFT_73,24174
14,NAPOLEONB_DRAFT_74,19891
14,NAPOLEONB_DRAFT_75,12457
14,NAPOLEONB_DRAFT_76,8135
14,NAPOLEONB_DRAFT_79,18042
14,NAPOLEONB_DRAFT_83,18383
14,NAPOLEONB_DRAFT_84,23835
14,NAPOLEONB_DRAFT_85,11536
14,NAPOLEONB_DRAFT_86,9686
14,NAPOLEONB_DRAFT_91,2726
14,NAPOLEONB_DRAFT_92,23725
14,NAPOLEONB_DRAFT_93,6962
15,NAPOLEONB_DRAFT_1,23265
15,NAPOLEONB_DRAFT_25,10017
15,NAPOLEONB_DRAFT_37,3102
15,NAPOLEONB_DRAFT_39,14856
16,NAPOLEONB_DRAFT_26,25449
17,NAPOLEONB_DRAFT_87,21252
19,NAPOLEONB_DRAFT_10,1045
20,NAPOLEONB_DRAFT_11,17557
21,NAPOLEONB_DRAFT_53,15168
21,NAPOLEONB_DRAFT_69,24820
23,NAPOLEONB_DRAFT_95,24540
24,NAPOLEONB_DRAFT_56,24917
32,NAPOLEONB_DRAFT_54,12742
36,NAPOLEONB_DRAFT_13,9387
37,NAPOLEONB_DRAFT_58,8182
38,NAPOLEONB_DRAFT_24,19229
38,NAPOLEONB_DRAFT_38,24858
41,NAPOLEONB_DRAFT_23,1881
46,NAPOLEONB_DRAFT_42,15962
46,NAPOLEONB_DRAFT_77,9657
47,NAPOLEONB_DRAFT_71,9303
56,NAPOLEONB_DRAFT_97,17850
62,NAPOLEONB_DRAFT_8,15225
83,NAPOLEONB_DRAFT_3,13517
84,NAPOLEONB_DRAFT_12,943
84,NAPOLEONB_DRAFT_2,2026
84,NAPOLEONB_DRAFT_44,24949
85,NAPOLEONB_DRAFT_100,24878
85,NAPOLEONB_DRAFT_15,12693
85,NAPOLEONB_DRAFT_19,21786
85,NAPOLEONB_DRAFT_20,17763
85,NAPOLEONB_DRAFT_5,4824
85,NAPOLEONB_DRAFT_61,19831
85,NAPOLEONB_DRAFT_62,19129
85,NAPOLEONB_DRAFT_7,9738
85,NAPOLEONB_DRAFT_81,24076
85,NAPOLEONB_DRAFT_9,16027
85,NAPOLEONB_DRAFT_98,20968
85,NAPOLEONB_DRAFT_99,1026
114,NAPOLEONB_DRAFT_14,4461
114,NAPOLEONB_DRAFT_16,4461

 

Next Steps:

Begin individual project

March 22

Individual Projects and Poster Reflection 3/20/19

Title: Individual Projects and Poster Reflection

Date: 20 March 2019

Rationale: The purpose of this lab was to reflect on the poster making process and begin formulating a question for a research project

Procedure:

  • Written reflection was done on the process of making the poster individually and as a group
  • Literature and tools were explored to begin formulating a question for the research project

Results/Observations:

  • Initial discussions yielded a common interest in attP sites and Start Site Commonality
  • More research and collaboration will be done to narrow this down

Next Steps/Conclusions:

  • More sources need to be consulted to fully formulate a question for the research project
  • The provided tools will be explored in order to discover more information on the eventual research project

 

March 22

3/18 ~ Final Edits of the Poster

Rationale: Listen to the critiques of the TA’s as well as Dr. Adair to finish the final poster

 

Materials:

  • Final Poster Google Document
  • Computer

 

Procedure:

  • Listened to the advice of the TA’s and Dr. Adair
  • Opened the Google doc and worked with the group to finish/clean up the wet-lab results section of the poster

 

Observations:

The Final Poster

 

Conclusion/Next Steps: After finishing up the poster, the cohort will begin to work on individual group projects.

March 22

Final Poster Editing 3/18/19

Title: Final Poster Editing

Date: 18 March 2019

Rationale: The purpose of this lab was to make any final edits that were needed to the class poster in order to make it ready for the scholars week presentation.

Tools:

  • Microsoft Surface Tablet
  • Google Slides

Procedure: 

  • Worked to create a figure encompassing wet lab results for NapoleonB
  • Worked with rest of the group to ensure the wet lab results were appropriately represented

Results/Observations:

  • Below is a picture of the created figure for metadata:

Conclusions/Next Steps:

  • Once all sections are edited and revised, the physical construction of the poster can be done and the poster will be ready to present
March 22

Creation of Final Poster and Start of Group Project (3/20/19)

Rationale:

Edits were made to finalize the poster, and groups were assigned to start the independent group project.

Tools:

  • PhagesDB
  • Google Slides

Procedure:

  1. Final edits and changes that need to be made were discussed as a class.
  2. Edits were implemented.
  3. Ideas about Group Project was discussed.

Results:

The final poster for URSA Scholars Week was edited and made.

Conclusion:

The poster will be complete to present for URSA Scholars Week by the end of this week. Ideas about the independent Group Project include creating a phylogenetic tree for phages.


Future Work:

Tools and research related to independent group project will be viewed and a idea will be chosen.

 

March 22

Edits for for URSA Scholars Week Poster (3/18/19)

Rationale:

Edits of Poster for URSA Scholars Week were implemented, images were added, and poster was formatted.

Tools:

  • Google Slides

Procedure:

  1. Changes to the poster were made as discussed in class.
  2. Each Part of the poster were edited by the different groups.
  3. Picture of Gel Electrophoresis was changed, a new infographic was added, and the overall text boxes were formatted.

Results:

A version of the final poster was created.

Conclusion:

The poster has not been finished, as some information needs to be inserted. Pictures were changed to represent accurate and current information.


Future Work:

The poster will be finished and  reviewed again, and required edits will be made.

March 22

Poster Final Check 3.20.19

Rationale:

To present the research, the lab members were divided into groups to discuss and design a poster, two groups were combined to produce the best parts of the two poster designs.

Materials:
  • Laptop
  • Google Slides
Procedure:

The advisors for the research projects were announced.

The potential gene 96-B was annotated and added to the in-silico results.

Results:

The poster design (without the in-silico results).

The Next Step:

The next step would be working on individual research projects with the assigned group.

March 22

Poster Layout Design 3.18.19

Rationale:

To present the research, the lab members were divided into groups to discuss and design a poster, two groups were combined to produce the best parts of the two poster designs.

Materials:
  • Laptop
  • Google Slides
Procedure:
  1. Work assignments were distributed among the class.
  2. Worked on the overall layout and design of the poster.
Results:

The Logos were swapped with versions with better resolutions, the font was changed to aerial and the text were aligned at the middle for most text boxes.

The Next Step:

The next lab would be the final poster design and the introduction to the individual research projects,

March 22

3.20.19 Final Check of Poster

Rationale:

To conduct a final check of the poster.

Procedure:

  1. I conducted a final check of the poster and fixed one error.
  2. I completed the QTM.
  3. We began looking at the independent research project.

Conclusion/Next Steps:

The poster should be free of layout and orientation errors and ready to be printed in that regard. The next step will be to continue working on our independent research project and presenting our poster at Scholars Week.