3/27/19

Rational:

To create a research question, to start looking at the literature relating to the question, and to look  into how to research the topic chosen.

Procedure:

• BLASTed NapoleonB’s endolysin and holin genes in order to see if they are conserved
• Searched for articles relating to holin proteins, endolysin proteins, and these protens in relation to phages
• Refined the topic on holin and endolysins in phage into a research question

Observations:

• The BLAST showed that both gene have a CDD and are conserved meaning that the genes will likely be similar to other phages
• The final queston was Using bioinformatics can phage’s endolysin and holin structures and functions compare to other Arthrobacter phages?

Conclusion:

By the end of lab we discovered many articles to use in order start our research. We also created the final research question and confirmed that we would be able to with our resources research the topic. Next lab we will prepare for the poster presentation for our class poster.