Rationale:

To understand more about phage, the class was divided into several small groups to generate research questions that utilize bioinformatic tools to learn more about questions about NapoleonB and/or related phages.

Materials:

• Laptop
• Canvas Bio-Lab info page
• Gepard dot plot software

Procedure & Results:

After looking for repeats in the NapoleonB genome, an outline of the question is generated,

Outline:

1.      Locate and identify and significant repeats in NapleonB’s genome
2.      Cross-check those repeats with other AM cluster phages
3.      BLASTn any significant repeats to check for similarity with NCBI database
4.      Check if any conclusions can be made with non-coding regions, protein similarity, NKF genes, etc.*

Tools*: Gepard, NCBI BLAST, DNA Master, Clustal Omega

*will be expanded

The Next Step:

The next step would be looking for initial points of interest in the NapoleonB genome, to find a incision point for the research to begin.