Rationale:

To understand more about phage, the class was divided into several small groups to generate research questions that utilize bioinformatic tools to learn more about questions about NapoleonB and/or related phages.

Materials:

• Laptop
• Canvas Bio-Lab info page

Procedure & Results:

Different aspects of possible research directions were explored and four questions were generated. The Gepard dot plot software was used to find repeats in the NapoleonB genome. The leading question for the group is:

Is it possible to use repeats in coding or noncoding regions of the genomes within the AM phage cluster to predict the functions of genes with no known functions?

The Next Step:

The next step would be trying to refine the question and have a clear and specific objective.