March 28

Start site preference of certian proteins 3/27

Print Friendly, PDF & Email

Rationale: Refine the question made on 3/25 and create a procedure to test the research question.

Procedure: Questions were refined and ideas were bounced from group members to coach. Performed NCBI BLASTp on a tape measure protein to see other know bacteria to have the same known protein, and the starts of AM cluster tape measure proteins were determined.

Results: The following images below show the NCBI BLASTp and confirmation that all AM cluster phages have methionine as the first amino acid sequence.

Conclusions: NCBI BLASTp showed many hits with many known bacteria to have the same protein meaning there are similarities between them.

Future work: Test to see if these hits have the same start sequences. Find a database that can allow the manipulation of the starts to see if the change of the start site of the tape measure protein has an effect on the shape of the protein. See if there is a correlation between the starts and why certain proteins prefer this start sequence rather than the other two start sequences.


Posted March 28, 2019 by michael_lum1 in category Michael Lum

Leave a Comment

Your email address will not be published. Required fields are marked *

*