Rationale

Create a independent research question that is related to the findings of Napoleon, be able to test it and analyze results.

Procedure

1. Created 4 questions
2. Choose one question that was approved by coach
3. Used NPS for multiple sequence alignment of different nicotinamide mononucleotide transporters
4. Use HHPred to analyze hits of the transporters that were not in AM cluster

Conclusion/Next Steps

Due to limited time, I was only able to analyze the sequence alignment from 6 genomes. Founded out the families and superfamily that the transporter was in (PnuC, SWEET, and TOG.) In the future, I would have to find more phages in the AM cluster and phages outside of the cluster that contain this transporter, take the genome sequence, analyze the alignment, and use HHpred to analyze the hits of those genomes.